Human Kallikrein 1 ELISA Kit (KLK1) (ab119598)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Kallikrein 1 ELISA Kit (KLK1)
See all Tissue kallikrein kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human KLK1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human KLK1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
A KLK1 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KLK1 is then added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human KLK1 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human Kallikrein 1 antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Human Kallikrein 1 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human Kallikrein 1 standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. -
Tissue specificity
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. - Information by UniProt
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Alternative names
- Glandular kallikrein 1
- hK 1
- hK1
see all -
Database links
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: P06870 Human
- Unigene: 123107 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab119598 has not yet been referenced specifically in any publications.