Human Kallikrein 1 ELISA Kit (KLK1) (ab119598)

Overview

  • Product name
    Human Kallikrein 1 ELISA Kit (KLK1)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human KLK1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human KLK1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

    A KLK1 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KLK1 is then added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human KLK1 amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab119598

Protocols

References

ab119598 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Detection of human KLK1 in saliva is likely but unconfirmed by experiment. The antibodies in the assay should be capable of recognizing both isoforms 1 and 2, which are described at the UniProt entry at this link. http://www.uniprot.org/uniprot/P06870....

Read More

This product will work for cell culture supernatants. Prepare samples as directed below: Cell culture supernates or tissue lysates: Remove particulates by centrifugation, assay immediately or aliquot and store samples at -20°C.

Thank you for your inquiry.

We have never tested this ELISA kit with urine samples (only serum, plasma, and tissue extracts) so we do not know if it will work. Theoretically if urine has KLK1 concentrations within the range of detection 156 p...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up