Human Kallikrein 1 ELISA Kit (KLK1) (ab119598)


  • Product nameHuman Kallikrein 1 ELISA Kit (KLK1)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Cell Lysate, Tissue Homogenate
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human KLK1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human KLK1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

    A KLK1 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KLK1 is then added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human KLK1 amount of sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionGlandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
  • Tissue specificityIsoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational
    The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located.
  • Information by UniProt
  • Alternative names
    • Glandular kallikrein 1
    • hK1
    • Kallikrein 1 renal/pancreas/salivary
    • Kallikrein serine protease 1
    • Kallikrein-1
    • Kidney/pancreas/salivary gland kallikrein
    • KLK1
    • KLK1_HUMAN
    • Klk6
    • KLKR
    • Tissue kallikrein
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Kallikrein 1 ELISA Kit (KLK1) images

  • Representative Standard Curve using ab119598


References for Human Kallikrein 1 ELISA Kit (KLK1) (ab119598)

ab119598 has not yet been referenced specifically in any publications.

Product Wall

Detection of human KLK1 in saliva is likely but unconfirmed by experiment. The antibodies in the assay should be capable of recognizing both isoforms 1 and 2, which are described at the UniProt entry at this link.

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This product will work for cell culture supernatants. Prepare samples as directed below: Cell culture supernates or tissue lysates: Remove particulates by centrifugation, assay immediately or aliquot and store samples at -20°C.

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We have never tested this ELISA kit with urine samples (only serum, plasma, and tissue extracts) so we do not know if it will work. Theoretically if urine has KLK1 concentrations within the range of detection 156 p...

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