Overview

  • Product name
    Human Kallikrein 11 ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 1399pg/ml 68.55 4.9%
    2 16 3520pg/ml 204.16 5.8%
    3 16 6635pg/ml 364.92 5.5%
    Inter-assay
    Sample n Mean SD CV%
    1 24 1575pg/ml 92.92 5.9%
    2 24 4236pg/ml 279.57 6.6%
    3 24 6751pg/ml 398.3 5.9%
  • Sample type
    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    The Human Kallikrein 11 (KLK11) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213801) is designed for the quantitative measurement of Human KLK11 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK11 amount of sample captured in plate.

  • Notes

    Kallikrein 11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Human KLK11 coated Microplate (12 x 8 wells) 1 unit
    Human KLK11 Standard (Lyophilized) 2 vials
    Biotinylated KLK11 Antibody 1 x 130µl
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Sample Diluent Buffer 1 x 30ml
    Antibody Diluent Buffer 1 x 12ml
    ABC Diluent Buffer 1 x 12ml
    TMB 1 x 10ml
    Stop solution 1 x 10ml
    Adhesive Plate Seal 4 units
  • Research areas
  • Function
    Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues.
  • Tissue specificity
    Expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 microg/mL (at protein level).
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications
    About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Hippostasin
    • hK11
    • Kallikrein 11
    • Kallikrein 11 isoform 1 preproprotein
    • Kallikrein 11 isoform 2 precursor
    • Kallikrein 11 precursor
    • Kallikrein related peptidase 11
    • Kallikrein-11 inactive chain 2
    • KLK 11
    • Klk11
    • KLK11_HUMAN
    • MGC33060
    • Protease serine 20 trypsin like
    • Protease serine trypsin like
    • PRSS20
    • Serine protease 20
    • TLSP
    • Trypsin like protease
    • Trypsin-like protease
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human KLK11 ELISA Kit (ab213801) Standard Curve.

Protocols

References

ab213801 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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