Overview

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab87266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-KCNN4 antibody (ab75956)

    Western blot

  • Purity70 - 90% by HPLC.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • hIKCa1
    • hKCa4
    • hSK4
    • IK1
    • IKCa1
    • Intermediate conductance calcium activated potassium channel protein 4
    • Intermediate conductance calcium-activated potassium channel protein 4
    • KCa3.1
    • KCa4
    • KCNN 4
    • Kcnn4
    • KCNN4_HUMAN
    • Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4
    • Putative Gardos channel
    • SK4
    • SKCa 4
    • SKCa4
    see all
  • FunctionForms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
  • Tissue specificityWidely expressed in non-excitable tissues.
  • Sequence similaritiesBelongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.
  • Post-translational
    modifications
    Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
  • Cellular localizationMembrane.
  • Information by UniProt

References for Human KCNN4 peptide (ab87266)

ab87266 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot

Review text: Samples used actually include porcine whole cell tissue and smooth muscle cell lysates.
Sample: Pig Tissue lysate - whole (Porcine Right Coronary Artery & Lung)
Loading amount: 20 µg
Specification: Porcine Right Coronary Artery & Lung
Gel Running Conditions: Non-reduced Non-Denaturing (Native) (Tris 4-20% Ready Gel)
Blocking step: Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details
Dilution: 1/200
Incubation time: 24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in TBS-Tween

Primary antibody (in addition to 'KCNN4 peptide')
Primary antibody: Abcam primary antibody: Anti-KCNN4 antibody (ab75956)
Dilution: 1/400

Secondary antibody
Name: Non-Abcam antibody was used: Donkey Anti-Rabbit IgG, Horseraddish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/2000

Detection
Detection method: ECL
Exposure: 1 minute(s) and 0 second(s)
Bands: Specific: ~60 kDa Non-specific: ~140 kDa

Additional data
Additional Notes: For WB image please Abreview for ab75956.
PVDF Membrane used
Lane 1 - Molecular Weight Marker
Lane 2- Porcine Right Coronary Artery (RCA) ab75956 only
Lane 3- Porcine Lung ab75956 only
Lane 4- Porcine Smooth Muscle Cells Control ab75956 only
Lane 5- Porcine Smooth Muscle Cells bFGF Treated ab75956 only
Lane 6 - Molecular Weight Marker
Lane 7- Porcine Right Coronary Artery (RCA) ab75956 + Peptide
Lane 8- Porcine Lung ab75956 + Peptide
Lane 9- Porcine Smooth Muscle Cells Control ab75956 + Peptide
Lane 10- Porcine Smooth Muscle Cells bFGF Treated ab75956+Peptide
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Submitted Jul 27 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"