Human kidney tissue lysate - insoluble fraction (male, 65 years) (ab44822)


  • Product nameHuman kidney tissue lysate - insoluble fraction (male, 65 years)
    See all Kidney lysates
  • Description
    Human Kidney insoluble fraction
  • General notes

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44822: Extraction 1: soluble protein fraction ab44820 Human Kidney Tumor Tissue lysate ab44816 Human Kidney Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44827 Human Kidney Tumor Tissue lysate ab44822 Human Kidney Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 0.1% SDS, PBS, 5.0M Urea, 2.0M Thiourea, 50mM DTT, pH 7.4
    Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human kidney tissue lysate - insoluble fraction (male, 65 years) (ab44822)

    ab44822 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I have had the opportunity to speak with the lab about your request. Unfortunately our lysates are not produced in a manner that would allow us to offer the proximal tubule as an isolate. We apologize for the inconvenience....

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