Human kidney tissue lysate (papillary renal cell carcinoma) - insoluble fraction (male, 65 years) (ab44827)

Overview

  • Product nameHuman kidney tissue lysate (papillary renal cell carcinoma) - insoluble fraction (male, 65 years)
    See all Kidney lysates
  • Description
    Human kidney insoluble fraction
  • General notesTissue: Kidney Location: Left kidney. Diagnosis: Papillary renal cell carcinoma. Stage: Not staged Grade: 1 Sex: Male Age: 65 years Gross findings: Tumor size 8 cm., well differentiated. Cut section pink. Encapsulated, hemorrhagic, cystic degeneration. Insoluble protein fraction.


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44827: Extraction 1: soluble protein fraction ab44820 Human Kidney Tumor Tissue lysate ab44816 Human Kidney Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44827 Human Kidney Tumor Tissue lysate ab44822 Human Kidney Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human kidney tissue lysate (papillary renal cell carcinoma) - insoluble fraction (male, 65 years) (ab44827)

    ab44827 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"