Human Kininogen ELISA Kit (KNG) (ab108875)

Overview

  • Product nameHuman Kininogen ELISA Kit (KNG)
    See all Kininogen kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    > 0.1 µg/ml
  • Range
    0.125 µg/ml - 4 µg/ml
  • Recovery

    97 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Kininogen (KNG) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kininogen in plasma and serum samples.


    A Kininogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Kininogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Kininogen captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human HMW Kininogen ELISA Kit (ab189574) from our SimpleStep ELISA® range.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • Function(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
  • Tissue specificitySecreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.
  • Involvement in diseaseDefects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.
  • Sequence similaritiesContains 3 cystatin domains.
  • Post-translational
    modifications
    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localizationSecreted > extracellular space.
  • Information by UniProt
  • Alternative names
    • Alpha-2-thiol proteinase inhibitor
    • BDK
    • BK
    • BK, included
    • bradykinin
    • bradykinin, included
    • Fitzgerald factor
    • Fitzgerald factor, included
    • Flaujeac factor, included
    • High molecular weight kininogen
    • High molecular weight kininogen, included
    • HMWK
    • HMWK, included
    • Ile-Ser-Bradykinin
    • Kallidin I
    • Kallidin II
    • kininogen 1
    • KNG
    • KNG1
    • KNG1_HUMAN
    • LMWK, included
    • Low molecular weight growth-promoting factor
    • Low molecular weight kininogen, included
    • Williams factor, included
    • Williams-Fitzgerald-Flaujeac factor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108875 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Human Kininogen ELISA Kit (KNG) images

  • Human Kininogen standard curve: mean of duplicates (+/- SD)
  • Kininogen measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 400-800 fold.

  • Representative Standard Curve using ab108875.

Protocols

References for Human Kininogen ELISA Kit (KNG) (ab108875)

ab108875 has not yet been referenced specifically in any publications.

Product Wall

Kininogen (KNG) Human ELISA Kit

Excellent Good 4/5 (Ease of Use)
Abreviews
Sample: EDTA human plasma stored at -80ºC
Amount of Sample: 3 ul
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 3 ul of plasma in 600 ul of 1XDiluent N (1:200)
Washings were done on a Microplate Washer machine with 300 ul of wash buffer 1X.
The standards data generated an excellent curve.
All samples assayed showed data inside the curve.
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Abcam user community

Verified customer

Submitted Dec 07 2015

Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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Vielen Dank für Ihre Anfrage. Ich entschuldige mich für die Wartezeit, ich musste auf nähere Angaben aus dem produzierenden Labor warten.

Ich freue mich Ihnen nun bestätigen zu können, dass beide Standards aus aufgere...

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Thank you for your reply.

I believe bulk discounts begin at 5 items per order. For a specific quote, please send your request to mailto:sales@abcam.com and one of our Sales Representatives will be able to help you with discounts.

I ...

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Thank you for your reply.

The source of this kit is considered proprietary, so I apologize that we are not able to provide this information.

They did release that the antigen for the antibodies in the kitis a full length protein Kin...

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Thank you for your inquiry.

The two antibodies in this kit are polyclonal and therefore will have multiple binding epitopes. The supplying lab does not have any information about the immunogen details for these antibodies.

I am sorr...

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Thank you for your email.

The antibody in the kit was raised against full length Kiniogen. It will react to multiple epitopes in the protein.

I hope this information helps. Please contact us with any other questions.

Thank you for contacting us.

Kate is currently away from the office but I will try to help as best I can with your enquiry. Firstly I am sorry for the delay in getting back to you. If the lot number of the kit your customer has been using is...

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I would like to thank you for noticing this and bringing it to our attention.

It appears in this case that there has been a mislabeled reagent for this kit. Our "Biotinylated Human Kininogen Antibody: 1 vial, lyophilized" should re...

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Thank you for contacting us regarding this. I have been in touch with the lab to try and clear this up.

This product is a competitive ELISA assay. The important thing is that Kininogen in your sample or in the standard is not biotinylated. O...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"