The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-KMT1B / SUV39H2 antibody (ab5264)
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
H3 K9 HMTase 2
Histone H3 K9 methyltransferase 2
Histone H3-K9 methyltransferase 2
Histone lysine N methyltransferase H3 lysine 9 specific 2
Histone lysine N methyltransferase SUV39H2
Histone-lysine N-methyltransferase SUV39H2
Lysine N methyltransferase 1B
Lysine N-methyltransferase 1B
sSuppressor of variegation 3 9 homolog 2 (Drosophila)
Su(var)3 9 Drosophila homolog of 2
Su(var)3 9 homolog 2
Su(var)3-9 homolog 2
Suppressor of variegation 3 9 homolog 2
Suppressor of variegation 3-9 homolog 2
FunctionHistone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher order chromatin organization during spermatogenesis.
Sequence similaritiesBelongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 chromo domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain.
DomainAlthough the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.
Cellular localizationNucleus. Chromosome > centromere. Associates with centromeric constitutive heterochromatin.