Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab71337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-KMT4 / Dot1L antibody (ab64077)

  • Purity
    70 - 90% by HPLC.

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Disrupter of telomere silencing protein 1
    • DOT 1
    • DOT1
    • DOT1 like
    • DOT1 like histone H3 methyltransferase
    • DOT1 like histone H3 methyltransferase (S. cerevisiae)
    • DOT1 like histone H3K79 methyltransferase
    • DOT1 like protein
    • DOT1, S. cerevisiae, homolog of
    • DOT1-like protein
    • DOT1L
    • DOT1L_HUMAN
    • EC 2.1.1.43
    • H3 K79 HMTase
    • H3 lysine-79 specific
    • H3-K79-HMTase
    • Histone H3 K79 methyltransferase
    • Histone H3 methyltransferase
    • Histone H3 methyltransferase DOT1-like
    • Histone H3-K79 methyltransferase
    • Histone lysine N methyltransferase H3 lysine 79 specific
    • Histone methyltransferase DOT1L
    • Histone-lysine N-methyltransferase
    • KIAA1814
    • KMT4
    • Lysine N methyltransferase 4
    • Lysine N-methyltransferase 4
    see all
  • Function
    Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
  • Sequence similarities
    Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
    Contains 1 DOT1 domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt

References

This product has been referenced in:
  • Ooga M  et al. Involvement of DOT1L in the remodeling of heterochromatin configuration during early preimplantation development in mice. Biol Reprod 89:145 (2013). Blocking . Read more (PubMed: 24132959) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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