Overview

Description

  • NatureSynthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab71337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-KMT4 / Dot1L antibody (ab64077)

  • Purity70 - 90% by HPLC.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • Disrupter of telomere silencing protein 1
    • DOT 1
    • DOT1
    • DOT1 like
    • DOT1 like histone H3 methyltransferase
    • DOT1 like histone H3 methyltransferase (S. cerevisiae)
    • DOT1 like histone H3K79 methyltransferase
    • DOT1 like protein
    • DOT1, S. cerevisiae, homolog of
    • DOT1-like protein
    • DOT1L
    • DOT1L_HUMAN
    • EC 2.1.1.43
    • H3 K79 HMTase
    • H3 lysine-79 specific
    • H3-K79-HMTase
    • Histone H3 K79 methyltransferase
    • Histone H3 methyltransferase
    • Histone H3 methyltransferase DOT1-like
    • Histone H3-K79 methyltransferase
    • Histone lysine N methyltransferase H3 lysine 79 specific
    • Histone methyltransferase DOT1L
    • Histone-lysine N-methyltransferase
    • KIAA1814
    • KMT4
    • Lysine N methyltransferase 4
    • Lysine N-methyltransferase 4
    see all
  • FunctionHistone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
  • Sequence similaritiesBelongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.
    Contains 1 DOT1 domain.
  • Cellular localizationNucleus.
  • Information by UniProt

References for Human KMT4 / Dot1L peptide (ab71337)

This product has been referenced in:
  • Ooga M  et al. Involvement of DOT1L in the remodeling of heterochromatin configuration during early preimplantation development in mice. Biol Reprod 89:145 (2013). Blocking . Read more (PubMed: 24132959) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab71337.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"