The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-KMT4 / Dot1L antibody (ab64077)
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Disrupter of telomere silencing protein 1
DOT1 like histone H3 methyltransferase
DOT1 like histone H3 methyltransferase (S. cerevisiae)
DOT1 like histone H3K79 methyltransferase
DOT1 like protein
DOT1, S. cerevisiae, homolog of
H3 K79 HMTase
H3 lysine-79 specific
Histone H3 K79 methyltransferase
Histone H3 methyltransferase
Histone H3 methyltransferase DOT1-like
Histone H3-K79 methyltransferase
Histone lysine N methyltransferase H3 lysine 79 specific
Histone methyltransferase DOT1L
Lysine N methyltransferase 4
Lysine N-methyltransferase 4
FunctionHistone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
Sequence similaritiesBelongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. Contains 1 DOT1 domain.
References for Human KMT4 / Dot1L peptide (ab71337)
This product has been referenced in:
Ooga M et al. Involvement of DOT1L in the remodeling of heterochromatin configuration during early preimplantation development in mice. Biol Reprod89:145 (2013).
Read more (PubMed: 24132959) »