Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab92421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-Ku70 antibody (ab83501)

  • Purity
    70 - 90% by HPLC.

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • 5''-deoxyribose-5-phosphate lyase Ku70
    • 5''-dRP lyase Ku70
    • 70 kDa subunit of Ku antigen
    • ATP dependent DNA helicase 2 subunit 1
    • ATP dependent DNA helicase II 70 kDa subunit
    • ATP-dependent DNA helicase 2 subunit 1
    • ATP-dependent DNA helicase II 70 kDa subunit
    • CTC box binding factor 75 kDa subunit
    • CTC box-binding factor 75 kDa subunit
    • CTC75
    • CTCBF
    • DNA repair protein XRCC6
    • G22P1
    • Ku 70
    • Ku autoantigen p70 subunit
    • Ku autoantigen, 70kDa
    • Ku p70
    • Ku70
    • Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa
    • Kup70
    • Lupus Ku autoantigen protein p70
    • ML8
    • Thyroid autoantigen
    • Thyroid autoantigen 70kD (Ku antigen)
    • Thyroid lupus autoantigen
    • Thyroid lupus autoantigen p70
    • Thyroid-lupus autoantigen
    • TLAA
    • X ray repair complementing defective repair in Chinese hamster cells 6
    • X-ray repair complementing defective repair in Chinese hamster cells 6
    • X-ray repair cross-complementing protein 6
    • XRCC 6
    • Xrcc6
    • XRCC6_HUMAN
    see all
  • Function
    Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
  • Sequence similarities
    Belongs to the ku70 family.
    Contains 1 Ku domain.
    Contains 1 SAP domain.
  • Developmental stage
    Expression does not increase during promyelocyte differentiation.
  • Post-translational
    modifications
    Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt

References

ab92421 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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