The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-Ku80 antibody (ab48013)
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
86 kDa subunit of Ku antigen
ATP dependent DNA helicase 2 subunit 2
ATP dependent DNA helicase II
ATP dependent DNA helicase II 80 kDa subunit
ATP dependent DNA helicase II 86 Kd subunit
ATP-dependent DNA helicase 2 subunit 2
ATP-dependent DNA helicase II 80 kDa subunit
CTC box binding factor 85 kDa
CTC box-binding factor 85 kDa subunit
DNA repair protein XRCC5
Ku autoantigen 80kDa
Ku86 autoantigen related protein 1
Lupus Ku autoantigen protein p86
Nuclear factor IV
Thyroid lupus autoantigen
X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining)
X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
X-ray repair cross-complementing protein 5
Xray repair complementing defective repair in Chinese hamster cells 5
FunctionSingle stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Sequence similaritiesBelongs to the ku80 family. Contains 1 Ku domain.
Developmental stageExpression increases during promyelocyte differentiation.
DomainThe EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.
Post-translational modificationsPhosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. Sumoylated.