Overview

Description

  • NatureSynthetic

Specifications

Our Abpromise guarantee covers the use of ab59815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking

  • Purity70 - 90% by HPLC.

  • FormLiquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • FLJ13161
    • Kinase phosphorylated during mitosis protein
    • KPM
    • Large tumor suppressor homolog 2
    • Large tumor suppressor homolog 2 Drosophila
    • Large tumor suppressor kinase 2
    • LATS large tumor suppressor Drosophila homolog 2
    • LATS large tumor suppressor homolog 2
    • Lats2
    • LATS2_HUMAN
    • Serine/threonine kinase kpm
    • Serine/threonine protein kinase kpm
    • Serine/threonine-protein kinase kpm
    • Serine/threonine-protein kinase LATS2
    • Warts like kinase
    • Warts-like kinase
    see all
  • FunctionNegative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.
  • Tissue specificityExpressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.
  • Post-translational
    modifications
    Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.
  • Information by UniProt

References for Human LATS2 peptide (ab59815)

ab59815 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"