|Sample type||Average %||Range|
|Cell culture supernatant||96.47||85% - 104%|
|Serum||95.29||84% - 103%|
|Plasma||94.68||83% - 103%|
Abcam’s LEC (CCL16) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LEC in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human LEC coated on a 96-well plate. Standards and samples are pipetted into the wells and LEC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LEC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LEC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human LEC||2 vials|
|LEC Microplate (12 x 8 wells)||1 unit|
|Recombinant Human LEC Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100532 has not yet been referenced specifically in any publications.