Human Anti-Leishmania IgG ELISA Kit (ab108749)

Overview

  • Product name
    Human Anti-Leishmania IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 4 5.9%
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Leishmania IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Leishmania in Human serum and plasma.

     

    A 96-well plate has been precoated with Leishmania antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Leishmania-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leishmania IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Protocols

    References for Human Anti-Leishmania IgG ELISA Kit (ab108749)

    ab108749 has not yet been referenced specifically in any publications.

    Product Wall

    This product has been tested with L.infantum in human serum or plasma collected with citrate. Other Leishmania species hasn’t been tested so we are unsure how well this kit will perform. In case you are interested in buying and trying this produc...

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