Human Anti-Leishmania IgG ELISA Kit (ab108749)

Overview

  • Product nameHuman Anti-Leishmania IgG ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Pos.Serum 7 7.4%
    Inter-assay
    Sample n Mean SD CV%
    Pos.Serum 4 5.9%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Leishmania IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Leishmania in Human serum and plasma.

     

    A 96-well plate has been precoated with Leishmania antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Leishmania-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Leishmania IgG sample captured in plate.

  • Tested applicationsSuitable for: Indirect ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Washing Solution White cap 1 x 50ml
    Cover foil 1 unit
    IgG Sample Diluent Yellow, White cap 1 x 100ml
    Leishmania (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 1 unit
    Leishmania IgG Cut-off Control Yellow, Green cap 1 x 3ml
    Leishmania IgG Negative Control Yellow, Blue cap 1 x 2ml
    Leishmania IgG Positive Control Yellow, Red cap 1 x 2ml
    Leishmania Protein A Conjugate Blue, Black cap 1 x 20ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution Yellow cap 1 x 15ml
  • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab108749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent dilution.

    Protocols

    References for Human Anti-Leishmania IgG ELISA Kit (ab108749)

    ab108749 has not yet been referenced specifically in any publications.

    Product Wall

    This product has been tested with L.infantum in human serum or plasma collected with citrate. Other Leishmania species hasn’t been tested so we are unsure how well this kit will perform. In case you are interested in buying and trying this produc...

    Read More

    Thank you for your enquiry.


    Since Iraq is a sanctioned country by the USA, I am unable to provide you product information or sell you any products. All Abcam offices abide by these same restrictions.

    Thank you for your understand...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"