Overview

  • Product name

    Human LIGHT ELISA Kit

  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 15 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.25 86% - 105%
    Plasma 97.63 85% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s LIGHT Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIGHT in plasma, and cell culture supernatants. (Human LIGHT concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human LIGHT coated on a 96-well plate. Standards and samples are pipetted into the wells and LIGHT present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIGHT antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIGHT bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human LIGHT in normal serum with ab100583, only in serum spiked with Human LIGHT.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human LIGHT 2 vials
    LIGHT Microplate (12 x 8 wells) 1 unit
    Recombinant Human LIGHT Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus.
  • Tissue specificity
    Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    modifications
    N-glycosylated.
    The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
  • Cellular localization
    Cytoplasm; Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • CD258
    • Delta transmembrane LIGHT
    • Herpes virus entry mediator ligand
    • Herpesvirus entry mediator A
    • Herpesvirus entry mediator ligand
    • HVEM L
    • HVEM-L
    • HVEML
    • Ligand for herpesvirus entry mediator
    • LTg
    • soluble form
    • TNF14_HUMAN
    • Tnfsf14
    • Tumor necrosis factor (ligand) superfamily member 14
    • Tumor necrosis factor ligand superfamily member 14
    • Tumor necrosis factor receptor like 2
    • Tumor necrosis factor superfamily member LIGHT
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100583.

  • Representative Standard Curve using ab100583.

Protocols

References

ab100583 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. I can confirm that the ab100583 LIGHT Human ELISA Kit is QC tested with EDTA, heparin and citrate anti-coagulants so all 3 will be compatible.  Other anticoagulants have not been tested. I hope this will be helpful t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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