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|Sample type||Average %||Range|
|Cell culture supernatant||95.06||74% - 107%|
|Serum||83||74% - 92%|
|Plasma||79.65||71% - 86%|
Abcam’s LIMP II Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LIMP-II in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human LIMP II coated on a 96-well plate. Standards and samples are pipetted into the wells and LIMP II present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LIMP II antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LIMP II bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|200X HRP-Streptavidin Concentrate||1 x 200µl|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Human LIMP II||2 vials|
|LIMP II Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human LIMP II Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab113336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab113336 has not yet been referenced specifically in any publications.