Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 32 years) (ab44478)

Overview

  • Product nameHuman liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 32 years)
    See all Liver lysates
  • Description
    Human liver insoluble fraction lysate
  • General notes

    Tissue: Liver Location: Liver Diagnosis: Hepatocellular carcinoma, moderately differentiated Stage: II T2N0MX Grade: II Macroscopic findings: Tumor size 2.5cm. Cut section homogenous, friable and brown. Size: 2.5 cm. Color: Brown, Consistency: Friable, Cut surface: Homogenous Presence of Necrosis and Lymphocytic infiltration. Cellular differentiation: glandular, with cell stratification and glandular formation. Presence of nuclear atypia.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44478: Extraction 1: soluble protein fraction ab44476 Human Liver Tumor Tissue lysate ab44475 Human Liver Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44478 Human Liver Tumor Tissue lysate ab44477 Human Liver Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 32 years) (ab44478)

    ab44478 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"