Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 55 years) (ab44566)


  • Product nameHuman liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 55 years)
    See all Liver lysates
  • Description
    Human liver insoluble fraction lysate
  • General notes

    Tissue: Liver Location: Liver, right lobe Diagnosis: Hepatocellular carcinoma Stage: Not recorded. Grade: 2 Sex: Male Age: 55 years Appearance: Macroscopic Tumor is 10 cm in diameter, well demarcated. Cut section is soft, hemorrhagic and gray-white in color. Microscopic Tissue sections show proliferation of malignant epithelial cell clusters with round nuclei, predominant nucleoli and abundant cytoplasm. Nuclear chromatin is coarse and irregularly distributed. Mitoses are evident. The surrounding stroma shows hyperplastic capillaries and infiltration of lymphocytes. Necroses and blood vessel invasion not revealed.


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44566: Extraction 1: soluble protein fraction ab44564 Human Liver Tumor Tissue lysate ab44563 Human Liver Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44566 Human Liver Tumor Tissue lysate ab44565 Human Liver Normal Tissue lysate (matched)


  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 55 years) (ab44566)

    ab44566 has not yet been referenced specifically in any publications.

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