Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 61 years) (ab44490)

Overview

  • Product nameHuman liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 61 years)
    See all Liver lysates
  • Description
    Human liver insoluble fraction lysate
  • General notes

    Tissue: Liver Location: Right lobe Diagnosis: Hepatocellular carcinoma, moderately differentiated Stage: I Grade: II Gross findings: Tumor size 2cm, dark red in color. Cut section soft, hemorrhagic and white/gray.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44490: Extraction 1: soluble protein fraction ab44488 Human Liver Tumor Tissue lysate ab44487 Human Liver Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44490 Human Liver Tumor Tissue lysate ab44489 Human Liver Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male, 61 years) (ab44490)

    ab44490 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"