Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male) (ab44486)

Overview

  • Product nameHuman liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male)
    See all Liver lysates
  • Description
    Human liver insoluble fraction lysate
  • General notesTissue: Liver Location: Right lobe Diagnosis: Hepatocellular carcinoma, poorly differentiated Stage: not recorded Grade: III Sex: Male Age: 3 months Gross findings: Tumor size 4cm x 5cm x 5cm. Cut section soft, hemorrhagic and dark red. Histologic pattern: Cell distribution diffuse, mosaic, necrosis, lymphocytic infiltration, clusterized. Presence of cystic degeneration and bleeding. Cellular differentiation: adenomatous, with presence of glandular cell, cell stratification, secretion, intracellular vacuoles, and glandular formation. high nuclear atypia (nuclear grade: III) Insoluble protein fraction


    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44486: Extraction 1: soluble protein fraction ab44484 Human Liver Tumor Tissue lysate ab44483 Human Liver Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44486 Human Liver Tumor Tissue lysate ab44485 Human Liver Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human liver tissue lysate (hepatocellular carcinoma) - insoluble fraction (male) (ab44486)

    ab44486 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"