Human liver tissue lysate (hepatocellular carcinoma) - soluble fraction (male, 44 years) (ab44480)

Overview

  • Product nameHuman liver tissue lysate (hepatocellular carcinoma) - soluble fraction (male, 44 years)
    See all Liver lysates
  • Description
    Human liver soluble fraction lysate
  • General notes

    Tissue: Liver Location: Liver Diagnosis: Hepatocellular carcinoma, well differentiated Stage: III Grade: not recorded Gross findings: Tumor size 10cm by 15cm, ill demarcated. Cut section soft and ivory white. Microscopic findings: Tissue sections show proliferation of irregular malignant cells with clear cytoplasm with vacuoles. Nuclei have irregular nuclear membranes, cytoplasm is granular or vacuolated with large nuclei. Liver cells arranged in a trabecular pattern with anusocytoses, increased nucleo-cyotplasm ratio, hychomasia many mitoses.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44480: Extraction 1: soluble protein fraction ab44480 Human Liver Tumor Tissue lysate ab44479 Human Liver Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44482 Human Liver Tumor Tissue lysate ab44481 Human Liver Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    References for Human liver tissue lysate (hepatocellular carcinoma) - soluble fraction (male, 44 years) (ab44480)

    ab44480 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"