This product belongs to a set of lysates that were extracted from a specific tissue. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below products that relate to ab44591: Extraction 1: soluble protein fraction ab44589 Human Liver Normal Tissue lysate Extraction 2: insoluble protein fraction ab44591 Human Liver Normal Tissue lysate
Storage instructionsShipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.