Human lung tissue lysate (large cell carcinoma) - insoluble fraction (male, 61 years) (ab42202)

Overview

  • Product nameHuman lung tissue lysate (large cell carcinoma) - insoluble fraction (male, 61 years)
    See all Lung lysates
  • Description
    Human lung insoluble fraction lysate
  • General notes

    Tissue: Lung Location: Left lobe. Diagnosis: Large cell carcinoma , well differentiated. Stage: I Grade: III Gross findings: Tumor size 2 cm diameter. Cut section hard, ivory white. Microscopic findings: Tumor shows malignant cells. Malignant cells have clear cytoplasm with vacuoles. Nuclei have irregular nuclear membranes, cytoplasm is granular or vacuolated with large nucleoli. Many monstrous nuclei. The malignant cells have invaded into the stroma.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42202: Extraction 1: soluble protein fraction ab42200 Human Lung Tumor Tissue lysate ab42199 Human Lung Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42202 Human Lung Tumor Tissue lysate ab42201 Human Lung Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature

    References for Human lung tissue lysate (large cell carcinoma) - insoluble fraction (male, 61 years) (ab42202)

    ab42202 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"