Human lung tissue lysate - soluble fraction (male, 70 years) (ab42162)

Overview

  • Product nameHuman lung tissue lysate - soluble fraction (male, 70 years)
    See all Lung lysates
  • Description
    Human lung soluble fraction lysate
  • General notes

    Diagnosis: Normal 

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42162: Extraction 1: soluble protein fraction ab42163 Human Lung Tumor Tissue lysate ab42162 Human Lung Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42165 Human Lung Tumor Tissue lysate ab42164 Human Lung Normal Tissue lysate (matched)

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 0.0001% Pepstatin A, 0.018% Sodium orthovanadate, 0.004% Sodium fluoride, 0.0001% Aprotinin, 0.0001% Leupeptin, 0.25% Sodium deoxycholate, 0.017% PMSF, 0.1% SDS, 0.029% EDTA
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

    References for Human lung tissue lysate - soluble fraction (male, 70 years) (ab42162)

    ab42162 has not yet been referenced specifically in any publications.

    Product Wall

    I have received the information about the tissue lysates that I was waiting for from the laboratory that produces these for us.

    The supplier of the lung lysates is confident that their measurements of protein concentration are correct, and s...

    Read More

    Thank you for the blot images and protocol detail. This makes clear what you described with regards to the differences in signal intensity among the samples, all loaded at 20ug of protein per lane based on the datasheet protein concentration specificat...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"