Human lung tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 58 years) (ab42524)


  • Product nameHuman lung tissue lysate (squamous cell carcinoma) - insoluble fraction (male, 58 years)
    See all Lung lysates
  • Description
    Human lung insoluble fraction lysate
  • General notesTissue: Lung Location: Right lobe. Diagnosis: Squamous cell carcinoma Stage: I T1N0M0 Grade: II Sex: Male Age: 58 years Gross findings: Tumor size 5 cm diameter, well demarcated. Cut section soft, hemorrhagic and necrotic. Ipsilateral hilar lymph nodes:0 Microscopic findings: Tumor shows proliferation of malignant epithelial cell clusters. Epithelial cells form clusters with irregular, basophilic, large nuclei and predominant nucleoli. Nuclear chromatin is coarse and irregularly distributed. Mitotic figures are evident. The surrounding stroma is hemorrhagic and infiltrated by large numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Tumor necrosis and blood vessel invasion are revealed. Insoluble protein fraction.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42524: Extraction 1: soluble protein fraction ab42519 Human lung tumor tissue lysate ab42517 Human lung normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab42524 Human lung tumor tissue lysate ab42523 Human lung normal tissue lysate (matched)


  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Research areas

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