Overview

  • Product name
    Human Lymphotactin ELISA Kit
    See all Lymphotactin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 150 pg/ml
  • Range
    0.069 ng/ml - 50 ng/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 81.23 75% - 101%
    Serum 97.65 86% - 114%
    Plasma 72.19 68% - 109%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Lymphotactin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Lymphotactin in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Lymphotactin coated on a 96-well plate. Standards and samples are pipetted into the wells and Lymphotactin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Lymphotactin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lymphotactin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Function
    Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
  • Tissue specificity
    Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
  • Sequence similarities
    Belongs to the intercrine gamma family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • ATAC
    • C motif chemokine 1
    • Chemokine (C motif) ligand 1
    • Cytokine SCM-1
    • Cytokine SCM1
    • LPTN
    • LTN
    • Lymphotactin
    • Lymphotaxin
    • SCM 1
    • SCM 1 alpha
    • SCM-1-alpha
    • SCM1
    • SCM1 alpha
    • SCYC1
    • Small inducible cytokine C1
    • Small inducible cytokine subfamily C member 1
    • Small-inducible cytokine C1
    • XC chemokine ligand 1
    • Xcl1
    • XCL1_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Lymphotactin ELISA Kit images

  • Representative standard curve using ab100584

  • Representative standard curve using ab100584

Protocols

References for Human Lymphotactin ELISA Kit (ab100584)

ab100584 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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