Customer reviews and FAQs

Overview

  • Product nameHuman Lysozyme ELISA Kit (LZM)
    See all Lysozyme kits
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.7%
  • Sample type
    Milk
  • Assay typeCompetitive
  • Sensitivity
    = 0.09 µg/ml
  • Range
    0.093 µg/ml - 6 µg/ml
  • Recovery

    = 98 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.

    A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionLysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • Involvement in diseaseAmyloidosis 8
  • Sequence similaritiesBelongs to the glycosyl hydrolase 22 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • 1
    • 1 4 beta N acetylmuramidase C
    • 4-beta-N-acetylmuramidase C
    • EC 3.2.1.17
    • LYSC_HUMAN
    • Lysosyme
    • Lysozyme (renal amyloidosis)
    • Lysozyme C
    • Lysozyme C precursor
    • LYZ
    • LZM
    • Renal amyloidosis
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab108877 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent concentration.

    Human Lysozyme ELISA Kit (LZM) images

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108877.

    Protocols

    References for Human Lysozyme ELISA Kit (LZM) (ab108877)

    ab108877 has not yet been referenced specifically in any publications.

    Publishing research using ab108877? Please let us know so that we can cite the reference in this datasheet.

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    2 Abreviews or Q&A

    10
    Votes: Highest First

    Do you have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow?

    Thank you for your inquiry.
    Unfortunately, we do not have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow available in our catalog.
    I would like to recommend checking the Biocompare website ...

    Read More

    Inquiry: To whom it may concern, I recently used the lysozyme human ELISA kit for quantification in the apical washes of well-differentiated paediatric primary nasal epithelial cells (WD-PNECs), derived from nasal brushes. These cells are fully differe...

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    Thank you for contacting us.

    As suggested on the datasheet, I recommend diluting the samples 1:200 in Diluent. If necessary, further dilute samples within the range of 1:20 to 1:2000 into Diluent.

    I hope this information is helpful...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"