Overview

  • Product name
    Human Lysozyme ELISA Kit (LZM)
    See all Lysozyme kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.7%
  • Sample type
    Milk
  • Assay type
    Competitive
  • Sensitivity
    = 0.09 µg/ml
  • Range
    0.093 µg/ml - 6 µg/ml
  • Recovery

    = 98 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.

    A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108877.

Protocols

References

ab108877 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your inquiry.
Unfortunately, we do not have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow available in our catalog.
I would like to recommend checking the Biocompare website ...

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Thank you for contacting us.

As suggested on the datasheet, I recommend diluting the samples 1:200 in Diluent. If necessary, further dilute samples within the range of 1:20 to 1:2000 into Diluent.

I hope this information is helpful...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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