Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 4.5% |
Sample | n | Mean | SD | CV% |
---|---|---|---|---|
Overall | 8.7% |
= 98
%
Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.
Components | 1 x 96 tests |
---|---|
100X Streptavidin-Peroxidase Conjugate | 1 x 80µl |
10X Diluent M Concentrate | 1 x 30ml |
20X Wash Buffer Concentrate | 1 x 30ml |
Biotinylated Human Lysozyme (Lyophilized) | 1 vial |
Chromogen Substrate | 1 x 8ml |
Lysozyme Microplate (12 x 8 well strips) | 1 unit |
Lysozyme Standard | 1 vial |
Sealing Tapes | 3 units |
Stop Solution | 1 x 12ml |
Our Abpromise guarantee covers the use of ab108877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Competitive ELISA | Use at an assay dependent concentration. |
ab108877 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"