Overview

  • Product name
    Human Lysozyme ELISA Kit (LZM)
    See all Lysozyme kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.7%
  • Sample type
    Milk
  • Assay type
    Competitive
  • Sensitivity
    = 0.09 µg/ml
  • Range
    0.093 µg/ml - 6 µg/ml
  • Recovery

    = 98 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.

    A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108877.

Protocols

References

ab108877 has not yet been referenced specifically in any publications.

Publishing research using ab108877? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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2 reviews or Q&A

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Do you have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow?

Thank you for your inquiry.
Unfortunately, we do not have any human lysozyme antibodies that have been used as a pair in a sandwich ELISA or for lateral flow available in our catalog.
I would like to recommend checking the Biocompare website ...

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Inquiry: To whom it may concern, I recently used the lysozyme human ELISA kit for quantification in the apical washes of well-differentiated paediatric primary nasal epithelial cells (WD-PNECs), derived from nasal brushes. These cells are fully differe...

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Thank you for contacting us.

As suggested on the datasheet, I recommend diluting the samples 1:200 in Diluent. If necessary, further dilute samples within the range of 1:20 to 1:2000 into Diluent.

I hope this information is helpful...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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