Overview

  • Product nameHuman LYVE ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    8.19 pg/ml - 2000 pg/ml
  • Recovery

    78 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 76 69% - 85%
    Serum 76.38 67% - 87%
    Plasma 79.07 68% - 86%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human LYVE ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LYVE in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human LYVE coated on a 96-well plate. Standards and samples are pipetted into the wells and LYVE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LYVE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LYVE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionLigand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • Tissue specificityMainly expressed in endothelial cells lining lymphatic vessels.
  • Sequence similaritiesContains 1 Link domain.
  • Post-translational
    modifications
    O-glycosylated.
  • Cellular localizationMembrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • Alternative names
    • cell surface retention sequence binding protein-1
    • Cell surface retention sequence-binding protein 1
    • CRSBP-1
    • CRSBP1
    • extracellular link domain containing 1
    • extracellular link domain-containing 1
    • Extracellular link domain-containing protein 1
    • HAR
    • Hyaluronic acid receptor
    • lymphatic vessel endothelial hyaluronan receptor 1
    • Lymphatic vessel endothelial hyaluronic acid receptor 1
    • LYVE-1
    • LYVE1
    • LYVE1_HUMAN
    • XLKD1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human LYVE ELISA Kit images

  • Representative Standard Curve using ab100585.

  • Representative Standard Curve using ab100585.

  • ab100585 was used to detect LYVE in Human heparin plasma samples according to the kit protocol. 10 µL sample was used per well.

    This image is courtesy of an anonymous Abreview

    See Abreview

Protocols

References for Human LYVE ELISA Kit (ab100585)

ab100585 has not yet been referenced specifically in any publications.

Product Wall

Abreview for LYVE Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Heparin plasma)
Specification: Heparin plasma
Amount of Sample: 10 uL µg
Type of Kit Used: ELISA
Positive control: Kit standards
Did you use the Abcam Kit protocol?: Yes

Additional data
Abcam response: all good, good images
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Verified customer

Submitted Oct 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"