Overview

  • Product nameHuman Mac-2BP ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 11.9%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.92 ng/ml
  • Range
    12.5 ng/ml - 200 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 116 95% - 139%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Mac-2BP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Mac-2BP concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).

    Mac-2BP specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated Mac-2BP detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Mac-2BP amount of sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionPromotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
  • Tissue specificityUbiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
  • Sequence similaritiesContains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 1 SRCR domain.
  • Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • 90K
    • Basement membrane autoantigen p105
    • BTBD17B
    • Galectin-3-binding protein
    • L3 antigen
    • Lectin galactoside-binding soluble 3-binding protein
    • LG3BP_HUMAN
    • LGALS3BP
    • MAC 2 BP
    • Mac-2 BP
    • Mac-2-binding protein
    • MAC2BP
    • Serum protein 90K
    • Tumor-associated antigen 90K
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Mac-2BP ELISA Kit images

  • Representative Standard Curve using ab119502.

Protocols

References for Human Mac-2BP ELISA Kit (ab119502)

ab119502 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"