|Sample type||Average %||Range|
|Serum||116||95% - 139%|
Abcam’s Mac-2BP Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Mac-2BP concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).
Mac-2BP specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated Mac-2BP detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Mac-2BP amount of sample captured in plate.
|Components||1 x 96 tests|
|Adhesive Films||2 units|
|HRP-Conjugate anti-Mac-2BP monoclonal Antibody||1 x 13ml|
|Mac-2BP Standard 100ng/mL||1 x 1ml|
|Mac-2BP Standard 12.5ng/mL||1 x 1ml|
|Mac-2BP Standard 200ng/mL||1 x 1ml|
|Mac-2BP Standard 25ng/mL||1 x 1ml|
|Mac-2BP Standard 50ng/mL||1 x 1ml|
|Microplate coated with Anti-Human Mac-2BP monoclonal antibody (12 x 8 wells)||1 unit|
|Sample Diluent (10x)||1 x 15ml|
|Stop Solution||1 x 16ml|
|TMB Substrate Solution||1 x 12ml|
|Wash Buffer Concentrate (10x)||2 x 50ml|
Our Abpromise guarantee covers the use of ab119502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab119502 has not yet been referenced specifically in any publications.