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Product name Human Mac-2BP ELISA Kit
Detection method Colorimetric
Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
Assay type Sandwich (quantitative)
12.5 ng/ml -
Sample specific recovery
Assay duration Multiple steps standard assay
Abcam’s Mac-2BP Human
in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Mac-2BP concentrations in cell culture supernatant, serum and plasma (citrate, EDTA, heparin).
Mac-2BP specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated Mac-2BP detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Mac-2BP amount of sample captured in plate.
Tested applications Suitable for:
Sandwich ELISA more details
Platform Microplate Properties
Storage instructions Store at +4°C. Please refer to protocols.
1 x 96 tests
2 units HRP-Conjugate anti-Mac-2BP monoclonal Antibody
1 x 13ml Mac-2BP Standard 100ng/mL
1 x 1ml Mac-2BP Standard 12.5ng/mL
1 x 1ml Mac-2BP Standard 200ng/mL
1 x 1ml Mac-2BP Standard 25ng/mL
1 x 1ml Mac-2BP Standard 50ng/mL
1 x 1ml Microplate coated with Anti-Human Mac-2BP monoclonal antibody (12 x 8 wells)
1 unit Sample Diluent (10x)
1 x 15ml Stop Solution
1 x 16ml TMB Substrate Solution
1 x 12ml Wash Buffer Concentrate (10x)
2 x 50ml
Function Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.
Tissue specificity Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts.
Sequence similarities Contains 1 BACK (BTB/Kelch associated) domain. Contains 1 BTB (POZ) domain. Contains 1 SRCR domain.
Cellular localization Secreted. Secreted > extracellular space > extracellular matrix.
Information by UniProt
Basement membrane autoantigen p105
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Human Mac-2BP ELISA Kit images
Representative Standard Curve using ab119502.
References for Human Mac-2BP ELISA Kit (ab119502)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"