Human Macrophage Stimulating Protein ELISA Kit (ab100612)


  • Product nameHuman Macrophage Stimulating Protein ELISA Kit
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    8.23 pg/ml - 2000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 97.25 86% - 106%
    Serum 94.48 83% - 104%
    Plasma 95.29 82% - 105%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Macrophage Stimulating Protein Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Macrophage Stimulating Protein in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Macrophage Stimulating Protein coated on a 96-well plate. Standards and samples are pipetted into the wells and Macrophage Stimulating Protein present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Macrophage Stimulating Protein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Macrophage Stimulating Protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • RelevanceMacrophage Stimulating Protein contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.
  • Alternative names
    • D3F15S2
    • D3F15S2h
    • D9H3F15S2
    • DNF15S2
    • DNF15S2h
    • Hepatocyte growth factor like protein
    • Hepatocyte growth factor like protein homolog
    • HGFL
    • Macrophage stimulating 1
    • Macrophage stimulatory protein
    • MSP
    • MST1
    • NF15S2
    see all


Our Abpromise guarantee covers the use of ab100612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human Macrophage Stimulating Protein ELISA Kit images

  • Representative Standard Curve using ab100612.

  • Representative Standard Curve using ab100612.


References for Human Macrophage Stimulating Protein ELISA Kit (ab100612)

ab100612 has not yet been referenced specifically in any publications.

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Yes, EDTA, Heparin, or Citrate collected plasma can be used with ab100612.

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