|Sample type||Average %||Range|
|Cell culture supernatant||97.62||85% - 104%|
|Serum||92.65||80% - 102%|
|Plasma||90.42||79% - 101%|
Abcam’s MCP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP1 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human MCP1 ELISA Kit (CCL2) (ab179886) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human MCP1||2 vials|
|MCP1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MCP1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Human MCP1 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples diluted 2-10 fold.
Representative Standard Curve using ab100586
ab100586 has not yet been referenced specifically in any publications.