Overview

  • Product nameHuman MCP2 ELISA Kit
    See all MCP2 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1.5 pg/ml
  • Range
    0.82 pg/ml - 200 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.25 85% - 105%
    Serum 93.88 84% - 104%
    Plasma 91.63 82% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MCP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP2 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MCP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MCP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionChemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • Tissue specificityHighest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications
    N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C C motif chemokine 8
    • CCL 8
    • Ccl8
    • CCL8_HUMAN
    • Chemokine (C C motif) ligand 8
    • Chemokine ligand 8
    • HC14
    • MCP 2
    • MCP-2
    • MCP-2(6-76)
    • Monocyte chemoattractant protein 2
    • Monocyte chemotactic protein 2
    • SCYA10
    • SCYA8
    • Small inducible cytokine A8
    • Small inducible cytokine subfamily A (Cys Cys) member 8
    • Small-inducible cytokine A8
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human MCP2 ELISA Kit images

  • Representative Standard Curve using ab100587

  • Representative Standard Curve using ab100587

Protocols

References for Human MCP2 ELISA Kit (ab100587)

ab100587 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"