Abcam’s MCP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MCP2 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MCP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MCP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
Belongs to the intercrine beta (chemokine CC) family.
N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.