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|Sample type||Average %||Range|
|Cell culture supernatant||88.99||79% - 98%|
|Plasma||95.19||88% - 106%|
Abcam’s MCP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MCP3 in plasma, and cell culture supernatants. (Human MCP3 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human MCP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MCP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human MCP3 in normal serum with ab100588, only in serum spiked with Human MCP3.
Get higher sensitivity in only 90 minutes with Human MCP3 ELISA Kit (CCL7) (ab193769) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|240X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human MCP3||2 vials|
|MCP3 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MCP3 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100588 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100588 has not yet been referenced specifically in any publications.