Human MDC ELISA Kit (CCL22) (ab100591)


  • Product nameHuman MDC ELISA Kit (CCL22)
    See all MDC kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1.5 pg/ml
  • Range
    1.65 pg/ml - 400 pg/ml
  • Recovery

    82 %

    Sample specific recovery
    Sample type Average % Range
    Serum 83.18 74% - 98%
    Plasma 81.78 73% - 96%
    Cell culture extracts 82.65 73% - 97%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MDC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MDC in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MDC coated on a 96-well plate. Standards and samples are pipetted into the wells and MDC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MDC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MDC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate


  • FunctionMay play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • Tissue specificityHighly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • A-152E5.1
    • ABCD 1
    • ABCD1
    • C-C motif chemokine 22
    • CC chemokine STCP-1
    • Ccl22
    • CCL22_HUMAN
    • Chemokine (C-C motif) ligand 22
    • DC/B-CK
    • Macrophage-derived chemokine
    • MDC(1-69)
    • MDC(7-69)
    • MGC34554
    • SCYA22
    • Small inducible cytokine A22
    • Small inducible cytokine subfamily A (Cys-Cys), member 22
    • Small-inducible cytokine A22
    • STCP 1
    • STCP1
    • Stimulated T cell chemotactic protein 1
    • Stimulated T-cell chemotactic protein 1
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human MDC ELISA Kit (CCL22) images

  • Representative Standard Curve using ab100591.

  • Representative Standard Curve using ab100591.

  • Data shows secreation of MDC (CCL22) by human macrophages isolated by negative selection in culture for 3 days.

    Specificity shown by selective secretion by M2 macrophages (MSCF + IL4) compared to the others subtypes (M0 = MCSF, M1 = MSCF + IFNg, DEX = MCSF + Dexamethasone, IL10 = MCSF + IL10, TGFb = MCSF + TGFb).


References for Human MDC ELISA Kit (CCL22) (ab100591)

ab100591 has not yet been referenced specifically in any publications.

Product Wall

CCL22-MDC secretion by Human macrophages

Excellent Excellent 5/5 (Ease of Use)
CCL22-MDC secretion by human macrophages isolated by negative selection in culture for 3 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by selective secretion by M2 macrophages (MSCF+IL4) compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)

Abcam user community

Verified customer

Submitted Mar 12 2014

Thank you for contacting us.

We unfortunately do not have these kits available for human samples. I however have contacted the product manager and asked them if these products are in pipeline, I will get back to you once I get it confirmed. Read More