The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Purity70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Activator-recruited cofactor 250 kDa component
Mediator complex subunit 13
Mediator of RNA polymerase II transcription subunit 13 homolog
Mediator of RNA polymerase II transcription, subunit 13
Thyroid hormone receptor associated protein 240 kDa subunit
Thyroid hormone receptor associated protein complex component TRAP240
Thyroid hormone receptor-associated protein 1
Thyroid hormone receptor-associated protein complex 240 kDa component
Vitamin D3 receptor-interacting protein complex component DRIP250
RelevanceMED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery.
References for Human MED13 peptide (ab60791)
has not yet been referenced specifically in any publications.
Publishing research using ab60791? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"