The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
70 - 90% by HPLC.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
Alpha 1,3(6) mannosylglycoprotein
Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase
Alpha mannoside beta 1,6 N acetylglucosaminyltransferase
Beta 1,6 N acetyl glucosaminyltransferase
GlcNAc T V
Mannoside acetylglucosaminyltransferase 5
Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase
N acetylglucosaminyl transferase V
N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein
Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.