Human MIF ELISA Kit (ab100594)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MIF ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 6 pg/ml -
Range
8.23 pg/ml - 6000 pg/ml -
Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.68 83% - 103% Serum 93.48 82% - 102% Plasma 95.41 84% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s MIF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIF in serum, plasma (collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernatants.
This assay employs an antibody specific for Human MIF coated on a 96-well plate. Standards and samples are pipetted into the wells and MIF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human MIF 2 vials MIF Microplate (12 x 8 wells) 1 unit Recombinant Human MIF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
MIF is a proinflammatory cytokine involved in many inflammatory reactions and disorders. MIF (macrophage migration inhibitory factor) was one of the first cytokines to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. MIF is involved in both autoimmune disorders and tumorigenesis. -
Cellular localization
Secreted. Cytoplasm. Note: Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. -
Alternative names
- GIF
- GLIF
- Glycosylation inhibiting factor
see all -
Database links
- Entrez Gene: 4282 Human
- Omim: 153620 Human
- SwissProt: P14174 Human
Images
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MIF in THP1 supernatants from control cells or cells stimulated (P/L) for 24 hours with 50 ng x mL-1 PMA (ab120297) and 1 ug x mL-1 LPS for the last 6 hours. Samples tested in the range of 1/3-1/30 (duplicates, +/- SD).
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MIF measured in cell culture supernatants from control or treated (PHA: 2 days in 2% PHA-M; LifeTechnologies) PBMCs. Samples tested in the range of 1/3-1/30 (duplicates, +/- SD).
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MIF in human biological fluids (duplicates +/- SD). Data shown from undiluted serum, plasma and urine, and milk diuted 1/100-1/500. Mouse serum gave 0.12 ng/mL (duplicates) and no signal was detected in mouse plasma.
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Standard curve in assay buffer B with background signal subtracted (duplicates; +/- SD).
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Representative Standard Curve using ab100594.
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Representative Standard Curve using ab100594.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab100594 has been referenced in 2 publications.
- Qaisar R et al. Prediction of sarcopenia using a battery of circulating biomarkers. Sci Rep 11:8632 (2021). PubMed: 33883602
- Costa-Silva B et al. Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nat Cell Biol 17:816-26 (2015). PubMed: 25985394