Overview

  • Product nameHuman MIG ELISA Kit (CXCL9)
    See all CXCL9 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human CXCL9 (MIG) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human CXCL9 (MIG) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A CXCL9 (MIG) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for CXCL9 (MIG) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human CXCL9 (MIG) amount of sample captured in plate.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • C-X-C motif chemokine 9
    • Chemokine (C-X-C motif) ligand 9
    • Chemokine, CXC motif, ligand 9
    • CMK
    • Crg 10
    • CXCL9
    • CXCL9_HUMAN
    • Gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • Monokine induced by gamma interferon
    • Monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • Small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human MIG ELISA Kit (CXCL9) images

  • Representative Standard Curve using ab119588.

Protocols

References for Human MIG ELISA Kit (CXCL9) (ab119588)

This product has been referenced in:
  • Asemota OO  et al. Chemokines Responses to Ascaris Lumbricoides Sole Infection and Co-infection with Hookworm among Nigerians. N Am J Med Sci 6:84-8 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24695523) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"