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|Sample type||Average %||Range|
|Cell culture supernatant||125.2||120% - 132%|
|Serum||87.12||74% - 93%|
|Plasma||97.15||81% - 112%|
Abcam’s MIP1a (CCL3) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1a in serum, plasma (recommended using EDTA or Heparin plasma), and cell culture supernatants.
This assay employs an antibody specific for Human MIP1a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP1a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|400X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human MIP1a||2 vials|
|MIP1a Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MIP1a Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100596 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"