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|Sample type||Average %||Range|
|Cell culture extracts||89.75||78% - 102%|
|Tissue Extracts||93.28||81% - 105%|
Abcam’s MIP3a (CCL20) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3a in cell lysates and tissue lysates.
This assay employs an antibody specific for Human MIP3a coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|300X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|5X Sample Diluent Buffer||1 x 10ml|
|Biotinylated anti-Human MIP3a||2 vials|
|MIP3a Microplate (12 strips x 8 wells)||1 unit|
|Recombinant Human MIP3a Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100600 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100600 has not yet been referenced specifically in any publications.