Overview

  • Product nameHuman MIP3b ELISA Kit (CCL19)
    See all Macrophage Inflammatory Protein 3 beta kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2.05 pg/ml - 500 pg/ml
  • Recovery

    78 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 71.8 65% - 89%
    Serum 84.43 75% - 94%
    Plasma 79.88 71% - 90%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MIP3b Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP3b in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP3b coated on a 96- well plate. Standards and samples are pipetted into the wells and MIP3b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP3b antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionMay play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.
  • Tissue specificityExpressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Beta chemokine exodus 3
    • Beta-chemokine exodus-3
    • C C chemokine ligand 19
    • C-C motif chemokine 19
    • CC chemokine ligand 19
    • CCL 19
    • CCL19
    • CCL19_HUMAN
    • Chemokine (C C motif) ligand 19
    • Chemokine (CC motif) ligand 19
    • Chemokine C C Motif Ligand 19
    • Chemokine CC Motif Ligand 19
    • CK beta 11
    • CK beta-11
    • CKb 11
    • CKb11
    • EBI 1 ligand chemokine
    • EBI1 ligand chemokine
    • ELC
    • Epstein-Barr virus-induced molecule 1 ligand chemokine
    • Exodus 3
    • Exodus3
    • Macrophage inflammatory protein 3 beta
    • MGC34433
    • MIP 3 beta
    • MIP 3B
    • MIP-3-beta
    • MIP-3b
    • MIP3 beta
    • MIP3B
    • OTTHUMP00000000531
    • SCYA 19
    • SCYA19
    • Small inducible cytokine A19
    • Small inducible cytokine subfamily A (Cys Cys) member 1
    • Small-inducible cytokine A19
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human MIP3b ELISA Kit (CCL19) images

  • Representative Standard Curve using ab100601.

Protocols

References for Human MIP3b ELISA Kit (CCL19) (ab100601)

ab100601 has not yet been referenced specifically in any publications.

Product Wall

Thank you again for your email and for your patience while I have been in touch with the lab.



The lab has found that the standards are generally stable for short periods of time, but there isn't specific data regarding the standard...

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As you may know, this diluent does contain proteins and other solutes so it would not be uncommon to see some precipitation or cloudiness, especially if stored at low temperature and/or for a long period of ...

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Thank you for keeping me updated on the situation.

I've looked through our records and we haven't received reports from other users about high absorbance or kit-to-kit variability. I also looked through the orders sent to you, and all of the ...

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Thank you for your replies, and I'm glad to hear that the new kits have arrived.

All of the kits labelled "***"are from the same lot. The "-1, -2, -3" represent different assembly times for the kits. So, -1 kits were assem...

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Thanks for your reply and for keeping me updated.

I'll keep an eye out for your data, so you can send it whenever it's ready. I've asked the lab for some feedback on the issues you've noticed so far, and I'll get back to you once I hear from ...

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Thank you for keeping me updated, and I'm sorry to hear that the results haven't been as expected.

Do you have any data from these two partial runs that you could forward? I'm going to contact the lab to ask if they've noticed the precipitate...

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Thank you for your reply, and please let me know if you have any further questions or need anything else from me and I'll be happy to help.

Have a great day!

Thank you for contacting us with your questions, and for your patience while I've been in touch with the lab regarding this enquiry.

We also recommend starting with a 1:3 serum dilution, though the working dilution may need to be optimized fo...

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