Overview

  • Product nameHuman MIP4 ELISA Kit (CCL18)
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 2 pg/ml
  • Range
    2.74 pg/ml - 2000 pg/ml
  • Recovery

    96 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.61 86% - 105%
    Serum 95.74 83% - 103%
    Plasma 96.53 84% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MIP4 (CCL18) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MIP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSuitable for: Sandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionChemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
  • Tissue specificityExpressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
  • Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Alternative macrophage activation associated CC chemokine 1
    • Alternative macrophage activation-associated CC chemokine 1
    • AMAC 1
    • AMAC-1
    • AMAC1
    • C C motif chemokine 18
    • CC chemokine ligand 18
    • CC chemokine PARC
    • CCL18
    • CCL18(4-69)
    • CCL18_HUMAN
    • Chemokine (C C motif) ligand 18
    • Chemokine (C C) dendritic
    • Chemokine ligand 18
    • CKb7
    • DC CK1
    • DC-CK1
    • DCCK1
    • Dendritic cell chemokine 1
    • Macrophage inflammatory protein 4
    • MIP 4
    • MIP-4
    • MIP4
    • PARC
    • Pulmonary and activation regulated chemokine
    • Pulmonary and activation-regulated chemokine
    • Small inducible cytokine A18
    • Small-inducible cytokine A18
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Human MIP4 ELISA Kit (CCL18) images

  • MIP4 measured in human biological fluids; signals in rat and mouse samples were below level of detection (duplicates +/- SD).

  • Data shows secretion of MIP4 (CCL18) by human macrophages isolated by negative selection in culture for 3 days.
    Specificity shown by selective secretion by M2a macrophages (MSCF + IL4) and M2c (MCSF + IL10) compared to the others subtypes (M0 = MCSF, M1 = MSCF + IFNg, DEX = MCSF + Dexamethasone, TGFb = MCSF + TGFb).

  • Representative Standard Curve using ab100620.

  • Representative Standard Curve using ab100620.

Protocols

References for Human MIP4 ELISA Kit (CCL18) (ab100620)

This product has been referenced in:
  • Miyake M  et al. Investigation of CCL18 and A1AT as potential urinary biomarkers for bladder cancer detection. BMC Urol 13:42 (2013). ELISA ; Human . Read more (PubMed: 24011266) »
  • Urquidi V  et al. CCL18 in a Multiplex Urine-Based Assay for the Detection of Bladder Cancer. PLoS One 7:e37797 (2012). ELISA ; Human . Read more (PubMed: 22629457) »

See all 2 Publications for this product

Product Wall

The Assay Diluent A will have a slight yellow haze colorization.

CCL18-MIP4 secretion by macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL18-MIP4 secretion by human macrophages isolated by negative selection in culture for 3 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by selective secretion by M2a macrophages (MSCF+IL4) & M2c (MCSF+IL10) compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethasone TGFb=MCSF+TGFb)
Username

Abcam user community

Verified customer

Submitted Mar 18 2014

Thank you for contacting us.

We unfortunately do not have these kits available for human samples. I however have contacted the product manager and asked them if these products are in pipeline, I will get back to you once I get it confirmed. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"