The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-MiTF antibody (ab20663)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Class E basic helix-loop-helix protein 32
Homolog of mouse microphthalmia
Microphthalmia associated transcription factor
Microphthalmia, mouse, homolog of
Microphthalmia-associated transcription factor
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
Involvement in disease
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance. Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness. Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
Belongs to the MiT/TFE family. Contains 1 basic helix-loop-helix (bHLH) domain.
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
All lanes : Anti-MiTF antibody (ab20663) at 1 µg/ml
Lane 1 : HeLa whole cell lysate Lane 2 : HeLa nuclear lysate Lane 3 : A431 whole cell lysate Lane 4 : HeLa whole cell lysate with Human MiTF peptide (ab21454) at 1 µg/ml Lane 5 : HeLa nuclear lysate with Human MiTF peptide (ab21454) at 1 µg/ml Lane 6 : A431 whole cell lysate with Human MiTF peptide (ab21454) at 1 µg/ml
Lysates/proteins at 1 µg/ml per lane.
Secondary Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution