Overview

  • Product name
    Human MMP1 ELISA Kit
    See all MMP1 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    24.69 pg/ml - 18000 pg/ml
  • Recovery

    98 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 98.66 90% - 106%
    Serum 97.78 88% - 106%
    Plasma 99.59 90% - 107%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Product overview

    Abcam’s MMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP1 pro and active forms in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for Human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • MMP1 measured in cell culture medium with background signal subtracted (duplicates +/- SD).

  • MMP1 measured in biological fluids with background signal subtracted (duplicates +/- SD).

  • Representative Standard Curve using ab100603.

Protocols

References

This product has been referenced in:
  • Dey S  et al. Matrix metalloproteinase-1 (MMP-1) Promoter polymorphisms are well linked with lower stomach tumor formation in eastern Indian population. PLoS One 9:e88040 (2014). Read more (PubMed: 24505369) »

See 1 Publication for this product

Customer reviews and Q&As

Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les ...

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Thank you for your inquiry.

For ab100603 lot GR47531-1, it was entered into stock in June 2011. We set an expiration guidelinefor 1 year from that point, so June 2012.

For ab100651 lot GR49880-1 was put into stock in July, Aug and O...

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Thank you and your customerfor taking the time to complete our questionnaire and contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided w...

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Thank you for contacting Abcam.

Unfortunately, to our knowledge ab100603, ab100607, and ab100605 have not been tested with monkey samples so there is no cross reactivity data available. However, I believe that MMPs are very well conserved amo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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