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|Sample type||Average %||Range|
|Cell culture supernatant||98.66||90% - 106%|
|Serum||97.78||88% - 106%|
|Plasma||99.59||90% - 107%|
Abcam’s MMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP1 pro and active forms in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.
This assay employs an antibody specific for Human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|440X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human MMP1||2 vials|
|MMP1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MMP1 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
MMP1 measured in cell culture medium with background signal subtracted (duplicates +/- SD).
MMP1 measured in biological fluids with background signal subtracted (duplicates +/- SD).
Representative Standard Curve using ab100603.