Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab39297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 0.025% Brij 35, 0.175M Sodium chloride, 5mM Calcium chloride, 10 mM MES, pH 5.5

General info

  • Alternative names
    • 27 kDa interstitial collagenase
    • CLG
    • CLGN
    • CLGN
    • CLGN
    • collagenase, fibroblast
    • collagenase, interstitial
    • Fibroblast collagenase
    • Fibroblast collagenase
    • Interstitial collagenase
    • Interstitial collagenase precursor
    • Interstitial collagenase precursor
    • Matrix metallopeptidase 1 (interstitial collagenase)
    • Matrix metalloprotease 1
    • Matrix Metalloproteinase 1
    • Matrix Metalloproteinase 1
    • Matrix metalloproteinase-1
    • MM01
    • MM01
    • MMP 1
    • MMP 1
    • MMP-1
    • MMP1
    • MMP1_HUMAN
    • OTTHUMP00000045866
    see all
  • FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt

Human MMP1 full length protein images

References for Human MMP1 full length protein (ab39297)

ab39297 has not yet been referenced specifically in any publications.

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