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|Sample type||Average %||Range|
|Cell culture supernatant||98.31||87% - 109%|
|Serum||96.54||85% - 108%|
|Plasma||94.57||84% - 105%|
Abcam’s MMP10 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP10 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernates.
This assay employs an antibody specific for Human MMP10 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent.||1 x 15ml|
|600X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human MMP10||2 vials|
|MMP10 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MMP10 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100602 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100602 has not yet been referenced specifically in any publications.