|Sample type||Average %||Range|
|Cell culture supernatant||77.25||69% - 92%|
|Serum||81.27||70% - 93%|
|Plasma||84.53||72% - 95%|
Abcam’s Human MMP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP2 in serum, plasma, and cell culture supernates. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties).
This assay employs an antibody specific for Human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|440X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human MMP2||2 vials|
|MMP2 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MMP2 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
MMP2 measured in biological fluids showing quantity (ng) per mL of tested sample
Representative Standard Curve using ab100606.