Overview

  • Product name
    Human MMP3 ELISA Kit
    See all MMP3 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.3 ng/ml
  • Range
    0.41 ng/ml - 100 ng/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.72 84% - 103%
    Serum 95.39 83% - 103%
    Plasma 96.25 85% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP3 pro and active forms in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).


    This assay employs an antibody specific for Human MMP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get results in 90 minutes with Human MMP3 ELISA Kit (ab189572) from our SimpleStep ELISA® range.


     

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab100607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100607.

Protocols

References

ab100607 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abreview for MMP3 Human ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Human Cell (Skin fibroblasts)
Specification: Skin fibroblasts
Amount of Sample: 200 µg
Type of Kit Used: ELISA
Positive control: Included in kit as part of standard curve.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Incubation times are a little longer than in some other kits, which must be remembered when planning multiple experiments, but the data generated is of a better quality overall.

The graph shows the differing levels of MMP3 released by primary fibroblasts exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.

(Sorry I cant say what the actives actually are due to business nature of the work).
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Verified customer

Submitted Feb 09 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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