Overview

  • Product name
    Human MMP9 ELISA Kit (lyophilized)
    See all MMP9 kits
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.38 84% - 104%
    Serum 96.23 84% - 103%
    Plasma 94.64 83% - 102%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MMP9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP9 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for Human MMP9 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab100610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • MMP9 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab100610.

Protocols

References

This product has been referenced in:

See all 7 Publications for this product

Customer reviews and Q&As

Abreviews
75000 cells/well were seeded in a 6-well plate for 96 hours in serum-free medium with an induction with TNF-alpha alone or a mix of TNF-alpha and TGF-beta. Medium were collected and centrifuged at 2000 rpm for 10 minutes, then supernatants were transferred to 1.5 ml tubes and kept at -80°C until use.
The concentrations of MMP9 were quantified with commercially available ELISA kit (Abcam, catalog number ab100610) according to the manufacturer´s protocol without any dilution.
All samples were assayed in triplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale.
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Submitted Oct 16 2017

Abreviews
Uterine aspirates were collected by aspiration with a Cornier Pipelle (Eurogine Ref. 03040200) in the office of the clinician or in the operating room prior to surgery and transferred to 1.5 ml microtubes. Phosphate buffer saline was added in a 1:1 (v/v) ratio and centrifuged at 2,500 rcf for 20 min in order to separate the soluble fraction (supernatant) from the solid fraction (pellet). The supernatants were kept at -80°C until use.
The concentrations of MMP9 in the soluble fraction of uterine aspirates were quantified with commercially available ELISA kit (Abcam, catalog number ab100610) according to the manufacturer´s protocol . As no results are available regarding the levels of these proteins in uterine aspirates, samples were diluted using five serial dilutions: no dilution, 1:10, 1:100, 1:1000, 1:10000.
The same amount of total protein from 6 uterine aspirate samples was loaded in each well. All samples were assayed in duplicates using a microplate reader and values were reported as ng/mL. The mean absorbance for each set of duplicate standards and samples was calculated. The standard curve was plotted in a log10-log10 scale (see figure 1A). The CV (%) between the duplicates of the samples ranged from 0-13% (average of 4%). Concentration of MMP9 of uterine aspirates ranged from 1 to 664 ng/u (see figure 1B).
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Submitted Apr 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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