Overview

Description

  • NatureNatural
  • SourceNative
  • Amino Acid Sequence
    • SpeciesHuman
    • Amino acids0 to 0

Specifications

Our Abpromise guarantee covers the use of ab39308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • FormLiquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, PBS, 250mM Sodium chloride, pH 7.4

General info

  • Alternative names
    • 82 kDa matrix metalloproteinase-9
    • 92 kDa gelatinase
    • 92 kDa type IV collagenase
    • CLG 4B
    • CLG 4B
    • CLG4B
    • Collagenase Type 4 beta
    • Collagenase Type 4 beta
    • Collagenase type IV 92 KD
    • Collagenase type IV 92 KD
    • EC 3.4.24.35
    • Gelatinase 92 KD
    • Gelatinase 92 KD
    • Gelatinase B
    • Gelatinase beta
    • Gelatinase beta
    • GelatinaseB
    • GELB
    • GELB
    • Macrophage gelatinase
    • Macrophage gelatinase
    • MANDP2
    • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
    • Matrix Metalloproteinase 9
    • Matrix Metalloproteinase 9
    • MMP 9
    • MMP-9
    • MMP9
    • MMP9_HUMAN
    • Type V collagenase
    • Type V collagenase
    see all
  • FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
    -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • Tissue specificityProduced by normal alveolar macrophages and granulocytes.
  • Involvement in diseaseIntervertebral disc disease
    Metaphyseal anadysplasia 2
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin repeats.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
    N- and O-glycosylated.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Target information above from: UniProt accession P14780 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

References for Human MMP9 full length protein (ab39308)

This product has been referenced in:
  • Mishra PK  et al. MMP-9 gene ablation and TIMP-4 mitigate PAR-1-mediated cardiomyocyte dysfunction: a plausible role of dicer and miRNA. Cell Biochem Biophys 57:67-76 (2010). Functional Studies . Read more (PubMed: 20422465) »
  • Sans-Fons MG  et al. Matrix metalloproteinase-9 and cell division in neuroblastoma cells and bone marrow macrophages. Am J Pathol 177:2870-85 (2010). Functional Studies . Read more (PubMed: 20971732) »

See all 2 Publications for this product

Product Wall

1- DO i have to activate this product or the product can auto-activate once it is added to cell culture?
The product should be activated prior to use in functional studies.

2- Can i freeze the activated MMP or I have to use it right after ...

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The H-MMP-9 is a mixture of latent and active enzyme. On the average, about 25% of ab39308 was active. The concentration was lot dependent, and listed on the labels. The molecular weight of human MMP-9 is 92 kDa, so it should be easy to figure out the...

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Application Western blot

Sample: Human Purified protein (purified protein from abcam)
Loading amount: 0.06 µg
Specification: purified protein from abcam
Gel Running Conditions: Reduced Denaturing (7.5%)
Blocking step: Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Other product details
Dilution: 1/1000
Incubation time: 18 hour(s) and 0 minute(s) · Temperature: 4°C

Primary antibody (in addition to 'MMP9 protein (Human)')
Primary antibody: Abcam primary antibody: Anti-MMP9 antibody [56-2A4] (ab58803)
Dilution: 1/1000

Secondary antibody
Name: Non-Abcam antibody was used: Goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution: 1/200

Detection
Detection method: colorimetric
Exposure: 5 minute(s) and 0 second(s)
Bands: Specific: 97 kDa
Positive control: rast ovarian lysates
Username

Mrs. Henda Nabli

Verified customer

Submitted Jan 07 2013

Thank you for contacting us.

I have been in touch with the lab regarding this issue. We recommend using the following for activation of MMP9. Incubating with APMA (aminophenyl mercural acetate) at 1.0 mM for 30-60 minutes at 37 degrees Cel...

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Thank you for your reply.

Normally you would not be eligible to receive a discount code if you are using a credit note to pay for the initial purchase. In this case though, I can make an exception. Please proceed and place the order for ab393...

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Thank you very much for your interest in ab39308.

Although this product can be used for this application, we have not directly tested it, therefore, I can offer a discount off a future purchase if you buy ab39308 now, test it in gelatin gel ...

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MMP-9 has a propeptide domain that is cleaved just after the "Cysteine switch" region, releasing the first 100 residues.
The full length protein is 707 amino acids, with a predicted mass of 78.4 kDa, but glycosylation makes the 78 kDa protein
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Thank you for contacting Abcam.

The ab39308 protein is largely in the zymogen form. The enzyme does not need to be activated before running on an SDS PAGE substrate gel (gelatin zymogram), as the SDS acts to activate some of the MMP-9.
P...

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Thanks for your email.
I apologize for the delay. I am currently awaiting a reply from the lab that supplies us ab58803. I have contacted them again just now and encouraged them to reply as soon as possible.
Thanks for your patience. I will f...

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Thank you once again for your enquiries regarding our MMP9 proteins.

To follow up, I now have further information regarding the following proteins:

ab39308 MMP9 protein (Human)
ab39309 MMP9 protein (Mouse)
ab41067 MMP9 p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"